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Industrial Biotechnology and Biocatalysis Group

Iroon Polytechneiou 9, Zografou , Athens, 15780, Greece
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Lab Audience
  • Students
  • Teachers
  • Engineers
  • Researchers
  • Entrepeneurs
  • Makers
  • SME

Industrial Biotechnology and Biocatalysis (IndBioCat) group is part of the Biotechnology Laboratory (BL) that has a renowned world reputation in the area of biomass bioconversion and biochemistry of plant cell wall degrading enzymes for the production of bioactive compounds and biofuels. The last few years, IndBioCat has discovered novel enzymatic activities that can degrade or modify agroindustrial and food waste by screening/genome mining of Fusarium oxysporum and Myceliophthora thermophila. More specifically, the group has worked on the discovery of novel enzymes (cellulolytic, hemicellulolytic and ligninolytic enzymes) for the enzyme-aided extraction or modification of bioactive components (Biocatalysis in non-conventional media) from biomass using conventional and modern bioinformatics assisted strategies (genome mining), heterologous overexpression (Pichia pastoris, Escherichia coli) and biochemical characterization of carbohydrate degrading recombinant enzymes, study of the structure/function relationship and regulatory mechanisms of the enzymes induced by saprophytic organisms.
The available infrastructure of BL includes: Bioreactors of Solid and Submerged Culture (Laboratory and Pilot Scale), Biomolecule Purification - Recovery - Analysis Systems (Ultracentrifuge,Centrifuge, Chromatography-Electrophoresis), Analytical Devices (GC, HPLC, Spectrophotometers), PCR, Sterility Chambers for Cell Culture, Microbial Culture (Incubators) and Cell Disruption (Ultrasound, High-pressure Homogenizer) Devices, Ultrafiltration Systems, Spray and Freeze Dryers, Robust Computing and Bioinformatic Unit (Two Private Powerful Servers).
During the past few years, the IndBioCat has been involved in the superficial modification of textiles using enzymes to improve the final properties (hydrophilicity, dyeability) and recently, through the TCBL project and specifically through a collaboration with Athens’ Making Lab, it has been involved in dyeing textiles using bacterial pigments, which constitutes a sustainable process for the clothing industry (BioShades project).